Programme
Programme
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Schedule last updated: 11 November, 2024
Highlights of 2024
The role of PEF in enabling future food production systems, Leando Buchmann
Electrophysiological Effects of Electroporation on Myocardium, Quim Castellvi
Irreversible electroporation as an ablation technique, Rafael V. Davalos
Scaling non-viral gene delivery for cell based therapies, Paulo A. Garcia
Nucleic acid sensing, Loree Heller
Collateral effects of electroporation (heating, pain&contraction, electrochemistry), Antoni Ivorra
Challenges and limitations in high power pulse forming for electroporation, Vitalij Novickij
Real-time imaging and conductance tracking of individual electropores in live cells, Andrei Pakhomov
Pulsed field ablation treatment of cardiac arrhythmias, Vivek Reddy
Electroporation-based gene editing enables advanced adoptive cancer immuno-cellular therapeutics and next-generation smart-cells, David Sourdive
Preliminary programme
Electrical properties of cells and tissues in electric field
- Resting and induced transmembrane voltage
- Dielectric properties of biological tissues
- Temperature and conductivity changes in tissues
Physical chemistry of membrane electroporation
- Electrochemical membrane thermodynamics
- Molecular dynamics simulations
Electroporation in vitro and in vivo – protocols
- Electroporation of cells in vitro
- Electroporation of cells in vivo
Development of devices and electrodes
- Devices for in vitro and in vivo experimental work
- Electrodes for in vitro and in vivo
- Electroporation of cells in tissue – models of electroporation in vivo and their experimental validations
Electrochemotherapy of tumors
- Basic mechanisms of electrochemotherapy
- Preclinical studies
- Clinical use of electrochemotherapy
- Current developments and future perspectives
Application of electroporation in gene transfection
- Gene transfection in vitro
- Gene transfection in vivo
- Electroporation for gene therapy
- Electroporation for DNA vaccine
Laboratory and practical work
More details on the Labwork page (coming soon!)...
- Gene electrotransfer using locally enhanced electric field
- Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM
- Visualization of local ablation zone distribution between two needle electrodes
- Comparison of flow cytometry and spectrofluorometric measurements in cell permeabilization experiments
- Triggering action potential and electroporation in excitable cells exposed to electric pulses
- Eradication of antibiotic-resistant coli by the combination of antibiotics and electroporation in a continuous mode
- Analysis of electric field orientations on gene electrotransfer –visualization at the membrane level
- Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS
- Analysis of electric field orientations on gene electrotransfer efficiency
- Monitoring cell membrane depolarization due to electroporation using fluorescent plasma membrane potential indicator
- Impedance and texture analysis techniques for detecting and characterising electroporation in plant tissues
- Modelling, visualising, and tracking pH front formation during pulse delivery to agarose-based tissue phantoms
- Simultaneous measurement of sarcomere shortening and calcium transients in primary rat cardiomyocytes exposed to electroporation
- Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage and electrode position
- Numerical modeling of thermal effects during irreversible electroporation treatments
- Molecular dynamics simulations of membrane electroporation
- Measurement of electroporation pulses with oscilloscope, and voltage and current probes
- Development of pulsed power generators for electroporation
- Electroporation of cells and tissues – interactive e-learning course