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Labwork

Practical work

You will be able to choose practical work sessions only after you have paid the registration fee and have passed the laboratory safety test. Your chosen practical work preferences will be taken into account, until the places for practical work sessions are filled. Sessions with a limited number of participants will be filled on a first-come-first-served basis. Please be sure to mark all practical work sessions by priority, as practical work sessions have limited number of participants, therefore it’s possible that your second or third choice will be considered instead of your first one.


Looking for the practical work schedules? They are available below.

ON-SITE practical work schedule ONLINE practical work schedule


Laboratory safety test

You will be able to take laboratory safety test and select practical work sessions in lms.ebtt.org e-learning platform after registration. You should receive an e-mail with instructions how to access the e-learning platform.

S1 – Laboratory safety

S2 – Electroporation hardware safety

List of laboratory exercises

Below you can find a list of this year practical work sessions. We are offering on-site practical work sessions as well as interactive online training sessions for those who are participating EBTT online.

Laboratory exercises

Labwork On-site On-line
  L1 – Gene electrotransfer using locally enhanced electric field    
  L2 – Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM    
  L3 – Visualization of local ablation zone distribution between two needle electrodes    
  L4 – Comparison of flow cytometry and spectrofluorometric measurements in cell permeabilization experiments    
  L5 – Triggering action potential and electroporation in excitable cells exposed to electric pulses    
  L6 – Eradication of antibiotic-resistant E. coli by the combination of antibiotics and electroporation in a continuous mode    
  L7 – Analysis of electric field orientations on gene electrotransfer – visualization at the membrane level    
  L8 – Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS    
  L9 – Analysis of electric field orientations on gene electrotransfer efficiency    
  L10 – Monitoring cell membrane depolarization due to electroporation using fluorescent plasma membrane potential indicator    
  L11 – Impedance and texture analysis techniques for detecting and characterising electroporation in plant tissues    
  L12 – Modelling, visualising, and tracking pH front formation during pulse delivery to agarose-based tissue phantoms    
  L13 – Simultaneous measurement of sarcomere shortening and calcium transients in primary rat cardiomyocytes exposed to electrical pulses    

Computer modelling

Labwork On-site On-line
  C1 – Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage and electrode position    
  C2 – Numerical modeling of thermal effects during irreversible electroporation treatments    
  C3 – Molecular dynamics simulations of membrane electroporation    

Hardware development and measurement

Labwork On-site On-line
  H1 – Measurement of electroporation pulses with oscilloscope, and voltage and current probes    
  H2 – Development of pulsed power generators for electroporation    

E-learning

Labwork On-site On-line
  E1 – Electroporation of cells and tissues – interactive e-learning